Hemoglobin variant analysis (HPLC) Test In Ahmedabad

Hemoglobin variant analysis (HPLC) Test In Ahmedabad

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HPLC abbreviation is used to describe high-performance liquid chromatography. It is a method that is used to detect hemoglobin (Hb) variants. The mutant forms of hemoglobin, i.e. hemoglobin variants, are caused due to genetic variation. There are many commonly known variants. The sickle cell anemia, for instance, is responsible for causing disease.

HPLC is introduced to accurately diagnose hemoglobinopathies and thalassemia. This technique has the advantage of presenting excellent resolution, reproducibility and quantification of several normal and abnormal hemoglobin, which helps in accurate diagnosis of thalassemia syndromes. This test is performed in several places, as needed. You can also book the HPLC test in Ahmedabad online.

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About Hemoglobin variant analysis (HPLC) Test

Why is the HPLC test done?

The HPLC blood test helps to screen thalassemia and hemoglobinopathies. Hemoglobin variant analysis refers to the medical test that is associated or done to detect mainly two vital things-

  • the variant of hemoglobin in the blood
  • relevant changes in the genetic order or course, that is, the addition of genetic sequence

Of all the existing types of hemoglobin in the human body, the most common of forms are-

  • Hemoglobin A (Hb A)
  • Hemoglobin B (Hb A2), and
  • Hemoglobin F (Hb F).

Now, several forms or structures of hemoglobin that are inherited by the baby from parents might result in undefined blood disorders. These are defined as hemoglobin variants. The symptoms of having hemoglobin variants in the blood include weakness and fatigue, lack of energy, pale skin, jaundice, shortness of breathing, growth problems in children, etc.

What does the HPLC test measure?

Hemoglobin variant analysis (HPLC Test) helps to determine the genetic changes that appear in the alpha and beta-globin protein chains. Any type of genetic addition to the blood can be detected and depicted with the help of this test. However, this test cannot be included or combined with other blood tests, since it detects a rare or new hemoglobin variant in the blood.

On the other hand, this test also helps to determine whether the red blood cells are normal or there are any abnormalities in the sample of the blood.

Risk/side effects of Hemoglobin variant analysis-HPLC Test

A hemoglobin variant analysis-HPLC test is a simple blood test. This test involves no risks or side effects. Searching for “HPLC test near me” will bring you a plethora of options to choose the test from. However, a home collection for blood samples in Ahmedabad can be recommended, which will help you have the test at home.

You can check for HPLC blood test prices in Ahmedabad, which generally range from Rs. 800 up to Rs. 5,000. The HPLC blood test cost for home collection should be equal as well.


Why should you choose Flebo.in's home collection services?

Are you searching for an “HPLC test near me?” Flebo.in offers you a flexible procedure for booking your tests for home collection. There is a team of experts to guide you for each test, ensuring you get complete assistance during your online booking. Once you initiate a test reservation, one of the phlebotomists will arrive at the given time to collect your blood sample HPLC test in Ahmedabad.

The advantages of booking from Flebo.in are-


Satisfied Service: With an easy home-based service, you can enjoy flexibility and convenience.

Delivery of Report: Flebo.in promises timely delivery of your test reports.


Manage bookings: You can easily check and manage your bookings as well as maintain a history of your tests.


Customized time slots: Booking slots from 6 am till 9 pm available daily.

In the case of failing delivery on time, Flebo.in promises a refund guarantee.



How much does the HPLC test cost?

Depending on where you are opting for the service, the HPLC test cost may range from Rs. 1,000 to Rs. 5,000.


Is it mandatory to go for an HPLC blood test?

It is important to check the hemoglobin variant in your blood. It is not mandatory, but can be considered to be, since it helps to detect abnormalities in the blood.

Is there any Age Limit for the HPLC blood test?

The test can be recommended for all, however, aging adults should definitely consider getting it done, since they are prone to various defective health issues. This test is also suitable for pregnant women.

Interpreting Hemoglobin variant analysis-HPLC Test results

Interpreting Hemoglobin variant analysis-HPLC Test results

In the primary opinion, the reading of the test shows factors like retention time windows and area perfect. However, some geographical factors, clinical representation, etc were also taken into consideration. The test helps to interpret the following points:

  • Distribution of hemoglobin variants
  • Mean values of red cells parameters and fractions of hemoglobin variants

Generally, beta-thalassemia traits or BTT is found to be the most common hemoglobin variant, which accounts for 74.48%, along with an elevated HbA2 level, which is >3.5% and RT3.63-3.69 min. The table shows the accurate levels:

Peak name Area Elevated area RT
BTT  74.48% >3.5% 3.63-3.69

Overall, the result shows direct identification of hemoglobin variants with a high degree of precision in the quantification of minor or major, normal/abnormal, fractions of hemoglobin.

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Steps to book HPLC Blood Test For Home Collection in Ahmedabad

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