Hemoglobin variant analysis (HPLC) Test In Pune

Hemoglobin variant analysis (HPLC) Test In Pune

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HPLC abbreviation is used to describe high-performance liquid chromatography. It is a method that is used to detect hemoglobin (Hb) variants. The mutant forms of hemoglobin, i.e. hemoglobin variants, are caused due to genetic variation. There are many commonly known variants. The sickle cell anemia, for instance, is responsible for causing disease.

HPLC is introduced to accurately diagnose hemoglobinopathies and thalassemia. This technique has the advantage of presenting excellent resolution, reproducibility and quantification of several normal and abnormal hemoglobin, which helps in accurate diagnosis of thalassemia syndromes. This test is performed in several places, as needed. You can also book the HPLC test in Pune online.

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About Hemoglobin variant analysis (HPLC) Test

Why is the HPLC test done?

The HPLC blood test helps to screen thalassemia and hemoglobinopathies. Hemoglobin variant analysis refers to the medical test that is associated or done to detect mainly two vital things-

  • the variant of hemoglobin in the blood
  • relevant changes in the genetic order or course, that is, the addition of genetic sequence

Of all the existing types of hemoglobin in the human body, the most common of forms are-

  • Hemoglobin A (Hb A)
  • Hemoglobin B (Hb A2), and
  • Hemoglobin F (Hb F).

Now, several forms or structures of hemoglobin that are inherited by the baby from parents might result in undefined blood disorders. These are defined as hemoglobin variants. The symptoms of having hemoglobin variants in the blood include weakness and fatigue, lack of energy, pale skin, jaundice, shortness of breathing, growth problems in children, etc.

What does the HPLC test measure?

Hemoglobin variant analysis (HPLC Test) helps to determine the genetic changes that appear in the alpha and beta-globin protein chains. Any type of genetic addition to the blood can be detected and depicted with the help of this test. However, this test cannot be included or combined with other blood tests, since it detects a rare or new hemoglobin variant in the blood.

On the other hand, this test also helps to determine whether the red blood cells are normal or there are any abnormalities in the sample of the blood.

Risk/side effects of Hemoglobin variant analysis-HPLC Test

A hemoglobin variant analysis-HPLC test is a simple blood test. This test involves no risks or side effects. Searching for “HPLC test near me” will bring you a plethora of options to choose the test from. However, a home collection for blood samples in Pune can be recommended, which will help you have the test at home.

You can check for HPLC blood test prices in Pune, which generally range from Rs. 800 up to Rs. 5,000. The HPLC blood test cost for home collection should be equal as well.


Why should you choose Flebo.in's home collection services?

Are you searching for an “HPLC test near me?” Flebo.in offers you a flexible procedure for booking your tests for home collection. There is a team of experts to guide you for each test, ensuring you get complete assistance during your online booking. Once you initiate a test reservation, one of the phlebotomists will arrive at the given time to collect your blood sample HPLC test in Pune.

The advantages of booking from Flebo.in are-


Satisfied Service: With an easy home-based service, you can enjoy flexibility and convenience.

Delivery of Report: Flebo.in promises timely delivery of your test reports.


Manage bookings: You can easily check and manage your bookings as well as maintain a history of your tests.


Customized time slots: Booking slots from 6 am till 9 pm available daily.

In the case of failing delivery on time, Flebo.in promises a refund guarantee.



How much does the HPLC test cost?

Depending on where you are opting for the service, the HPLC test cost may range from Rs. 1,000 to Rs. 5,000.


Is it mandatory to go for an HPLC blood test?

It is important to check the hemoglobin variant in your blood. It is not mandatory, but can be considered to be, since it helps to detect abnormalities in the blood.

Is there any Age Limit for the HPLC blood test?

The test can be recommended for all, however, aging adults should definitely consider getting it done, since they are prone to various defective health issues. This test is also suitable for pregnant women.

Interpreting Hemoglobin variant analysis-HPLC Test results

Interpreting Hemoglobin variant analysis-HPLC Test results

In the primary opinion, the reading of the test shows factors like retention time windows and area perfect. However, some geographical factors, clinical representation, etc were also taken into consideration. The test helps to interpret the following points:

  • Distribution of hemoglobin variants
  • Mean values of red cells parameters and fractions of hemoglobin variants

Generally, beta-thalassemia traits or BTT is found to be the most common hemoglobin variant, which accounts for 74.48%, along with an elevated HbA2 level, which is >3.5% and RT3.63-3.69 min. The table shows the accurate levels:

Peak name Area Elevated area RT
BTT  74.48% >3.5% 3.63-3.69

Overall, the result shows direct identification of hemoglobin variants with a high degree of precision in the quantification of minor or major, normal/abnormal, fractions of hemoglobin.

Customer Reviews

Some of Flebo.in's customers reviews

Based on reviews
ravi rajravi raj
05:31 18 Jun 23
They are providing the best service. Sample was collected from my home at the fixed time with no delay. The Phlebo was very confident. Rates are very reasonable and minimum. I was surprised to get the report just after 24 hours and it was as per our expectations. Best Best.2
Mritunjay DwivediMritunjay Dwivedi
02:28 02 Jun 23
I am very happy to share that your service and staff are too good . I got my test done before mentioned time and It was very smooth. I am editing my review due to not getting my report on time. It has been 3 days but still I didn't get my report
Kanishka634 SharmaKanishka634 Sharma
03:43 28 May 23
Ms Sujata arrived on time and was very polite and caring throughout the process. Actually there was a mistake on my part where I couldn't attend her calls because I was not well and i feel asleep bcoz of my medicines. I had scheduled an appointment for my mother but Ms Sujata somehow reached my address by asking my neighbours. Such a lovely girl, and also the collection process was really smooth. Would absolutely recommend everyone to check out their services
Kawaljeet SinghKawaljeet Singh
02:35 28 May 23
Painless excellent service and she was kind hearted person love her way to treat us have a best future ahead to you miss Purnima g
mukesh dabramukesh dabra
07:33 09 May 23
Nice experience on having my regular routine blood check up from Flebo. Their technicians are also well qualified and well mannered to deal with. Kudos!
Anil AroraAnil Arora
14:38 21 Apr 23
Person who collects the sample are very expert in taking blood sample.Very good explanation of all test, a good Gentlemen.Test report/ results were sent in time.Even layman can understand the report before going to doctor for consultation.Always prefer flebo ,excellent service.
Anjali DubeyAnjali Dubey
02:46 05 Apr 23
Super!!! Rafi has collected very well. Very Nice with the whole protocol.With full protocal. He spoke very politely and collected! He had come on time.
Manthan VijManthan Vij
08:34 06 Mar 23
The experience was good and received reports within promised time. The sample collection was done smoothly with good care. Impressed and thank you so much to the whole team for the wonderful service.
Ashwin ShrivastavaAshwin Shrivastava
01:10 06 Mar 23
Great service Flebo mohit kumar whit out pain
02:26 25 Feb 23
Mr Pawan came to collect my sample, the person was so gentle n soft handed that I didn’t when he took my sample… kudos to the team.Amazing
Naman ArnejaNaman Arneja
07:10 23 Feb 23
They are providing the best service. Sample was collected from my home at the fixed time with no delay. The Phlebo was very confident. Rates are very reasonable and minimum. I was surprised to get the report just after 24 hours and it was as per our expectations. Best Best.
Abhishek SinghAbhishek Singh
04:03 02 Feb 23
My experience was really good with flebo. From the booking process till the end reports everything was very smooth .in such a less price service delivery was really good.Good and polite staff .
asim pashineasim pashine
05:58 24 Jan 23
Service provided by Flebo was great. My Sample Collection Person ( Mr. Shivam Prajapati ) did the process very smoothly and is of great patience. Have meet many Sample Collection Person but he was the best one. Seriously, next time also i prefer him to come for sample collection. ☺️☺️☺️☺️
chandan sharmachandan sharma
07:26 09 Jan 23
Our team is hard at work trying to continuously improve our services and it’s always rewarding to hear kind words.this services is excellent/good/osm/very nice..i'm very glad part of this company..
Vaibhav MittalVaibhav Mittal
03:21 21 Dec 22
The Flebo Javed was good at his job & also courteous & patient. The package I got was good too. Now I want to see how fast will I get the report. Will it be as promised on call, within 24 hrs or not. I will edit my review after receiving report.
sakshi palsakshi pal
08:41 19 Dec 22
My experience was really good with flebo. From the booking process till the end reports everything was very smooth .in such a less price service delivery was really good.Good and polite staff .I loved flebo's service ❤️
Kunal AtalKunal Atal
04:52 14 Nov 22
This is my first experience with Flebo and it was very nice and I highly impressed with the service.Sample collector boy came on time, very hygiene, took sample very nicely, polite speaking.The best part is company's sample collection kit. Everything is packed in a sealed packet and open in front of us.Call centre was also good and she suggested good to me.I really appropriate their quick response.I recommend it.Good job Flebo
Robin GoyaldzRobin Goyaldz
03:00 08 Nov 22
Happy from your service....sample collection boys are on time and while taking blood samples no pain an it was so quick and very nice way to talk ...me any my wife both are very happy from service ............. doing good for people at lover price. Thanks ????????
Pranav SinhaPranav Sinha
02:47 06 Nov 22
Good and quality service with qualified professional providing to their customers with maintain proper hygiene. Will recommend to go for this if needed any health check up.
Sushil MehlaSushil Mehla
08:10 04 Nov 22
Happy with flebo.in services. There way or representation and consulting to customer was absolutely amazing. It was my first experience with flebo.in. I interact with the flebo Ajay who guide me for suitable test and he was looking well experienced. Polite in behaviour and punctual. he gave me pain less experience while taking sample.Thank you for your service
anmol kohlianmol kohli
03:10 03 Nov 22
Best hygienic service provide well ☺️educated knowledgeable volunteers executive Flebo Company Affordable and accessible service provider, the employees of this company are very nice, 10 out of 10 points from me and my mom to this company to its employees. The staff who came were very well educated, intelligent,Keep working like this. ???? thanks best of luck.
amit Kumaramit Kumar
00:57 27 Oct 22
Very good experience..On timeGood staff
Ashok Kumar RaiAshok Kumar Rai
02:16 22 Oct 22
Excellent service by boy very good behaviour... God bless him
Bhawna SinghBhawna Singh
04:18 18 Oct 22
Excellent service ?..They arrived within a given time slot and they are polite and courteous and well qualified..
Abed 47Abed 47
03:34 17 Oct 22
Good service and experienced staff and we'll educatedFlebo ABED ALI Best phlebotomist, experience is awesome,

Steps to book HPLC Blood Test For Home Collection in Pune

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